Top Tip: How to defrost brains for dissection in 1 hour
This Top Tip comes from Patricia Hugman, Senior Laboratory Technician at St Joseph’s College Toowoomba. Thank you, Patricia! This is something we get asked all the time and the collective wisdom of lab techs is the best source of info.
Have you ever wondered how long it would take to defrost … brains for dissection?
I found the following method for defrosting brains to be very effective & efficient
To defrost brains ‘al dente’ in 1hr
1. Straight from freezer
2. Place brains in packet in suitable container & cover with water (4 litre icecream container works well) sitting ‘flat’ not ‘end on’
1pkt/ container defrosts faster that 2 in one container
3. @20min – Change water
4. @40min – discard water, remove brains from packet, sprinkle liberally with salt, just cover with water
5. @50min – gently separate brains, being careful of brainstem/medulla
6. @60min – carefully remove individual brains, pat dry with paper towel and ‘serve’ to students!
On a nice summer’s day (25deg C) this timing worked a treat. Producing brains that were still firm but not icy.
Sitting on the bench (in air) produced irregular defrosting >4hr
Got a Top Tip you are willing to share with other techs? Have a squeal to Miss Vivi and see your name in lights.
This entry was posted on February 24. 2014 by Miss Vivi
No Chemical Preservatives
We believe Lab Technicians and science Teachers are subjected to enough chemical exposure during their career as it is. Students, too, deserve to learn in an environment that limits their chemical exposure to those experiments that require the use of chemicals in a controlled and safe manner.
The specimens supplied by Dissection Connection are 100% chemical preservative free. No formalin, no glycol, no nothing. The exception are the small intestines which are packaged in a solution of sterile 0.9% saline to prevent dehydration of the specimen.
This entry was posted on February 18. 2014 by Miss Vivi
Securing stillborn piglet for dissection
The piglets are just too difficult to pin to a board in the tradition way for dissection. Heather from Southern Biological showed me this elastic band method between sessions at a conference one day.
This entry was posted on December 02. 2013 by Miss Vivi